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Its phenotype is + bio + th – liu – met.
Lederberg and Totem scattered the 2 mutants and
They have been skilled for a number of hours in a medium containing the addition of methionine, biotin, threonine and leucine.
Each strains can develop. Then the micro organism have been eliminated
Centrifugal, washed, changed by medium
In very small medium, it’s absent from all 4 enhances. pressure 1 or pressure 2 can’t develop on this medium
the diet they want. Nonetheless, some micro organism
Colonies appeared on tradition plates.
They’re small to medium, rising in these colonies.
+ Bio + Thr + Leu + should comprise micro organism discovered; car
Sure, they must be prototropic. These colonies first appeared at a fee of roughly one for each 10 million cells positioned within the tray (1/107).
The place did these prototropic colonies come from? Lederberg and Totem have been in a position to get rid of the mutation, and so forth. Investigators blocked this alteration (we mentioned this course of in Chapter 11, which we’ll take a look at in additional element
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